RESUMO
Background: testicular damage due to spermatic cord torsion may lead to infertility. It is probably because of changes in oxidative stress factors such as malondialdehyde
Objective: to investigate the protective effect of melatonin [MLT], as an antioxidant, on testicular damage induced by acute unilateral spermatic cord torsion and detorsion [T/D] in rats
Materials and Methods: in this experimental study, 48 adult male Wistar rats were randomly divided into three groups [8 rats/group]: sham group underwent right scrotal surgery only., the T/D group underwent right testicular torsion [for 1 hr] and detorsion, and the melatonin group underwent right testicular torsion, received 25 microg/kg melatonin intraperitoneally immediately after surgery of T/D. Then the histological parameters and malondialdehyde [MDA] changes were evaluated
Results: torsion and detorsion decreased the diameter of the tubules significantly compared to controls [p=0.003]. Melatonin could increase the diameter, but it was not significant [p=0.26]. The heights of the epithelium were constant in sham, T/D, and melatonin groups without any significant difference between groups [p=0.98]. Based on Johnsen's score, spermatogenesis was normal in the sham group. The torsion significantly injured all lineage cells [p<0.001]. There was no any spermatid or sperm in the seminiferous tubules. Melatonin improved the spermatogenesis significantly [p=0.02], but could not improve MDA level significantly [p=0.99]
Conclusion: severe degenerative changes of testis were induced by acute unilateral spermatic cord torsion and detorsion in rats, but it had no effect on MDA level
RESUMO
Deltamethrin [DM] is a synthetic pyrethroid insecticide that can elicit neurotoxicity, leading to apoptosis. There is accumulating evidence that oleuropein [OE] has anti-apoptotic effect. The purpose of this study was to determine the anti-apoptotic effect of OE pretreatment in the neuronal cells of cerebral cortex. Rats were randomly divided into four groups each containing five rats: DM-treated group [12.5 mg/kg, a single dose], OE-treated group [20 mg/kg per day], DM + OE-treated group, and vehicle group. Sections of the brain were obtained 24 hours after DM injection and studied for histopathological and immunohistochemistry assessment. The histopathological assessments showed lesser characteristics of neural degeneration in DM + OE group compared with DM group. Greater Bcl-2 and attenuated Bax expression could be detected in the DM + OE treatedmice compared with DM group. The results suggested that DM-induced neurotoxicity can be subsided by OE